Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Appl Environ Microbiol. 2012 May;78(10):3770-3. doi: 10.1128/AEM.00150-12. Epub 2012 Mar 9.
Cellobiose dehydrogenase (CDH) gene transcripts were quantified by reverse transcription-PCR (RT-PCR) in cultures of Phanerochaete chrysosporium supplemented with various cello- and xylooligosaccharides in order to elucidate the mechanism of enhanced CDH production in xylan/cellulose culture. Cellotriose and cellotetraose induced cdh expression, while xylobiose and xylotriose induced expression of cellobiohydrolase genes, especially cel7C.
通过在补充了各种纤维二糖和木低聚糖的黄孢原毛平革菌（Phanerochaete chrysosporium）培养物中进行反转录 PCR（RT-PCR），对纤维二糖脱氢酶（CDH）基因转录本进行了定量，以阐明在木聚糖/纤维素培养物中增强 CDH 产生的机制。纤维三糖和纤维四糖诱导 cdh 表达，而木二糖和木三糖诱导纤维素酶基因，特别是 cel7C 的表达。
