在担子菌门糙皮侧耳（Phanerochaete chrysosporium）中，纤维二糖脱氢酶基因对纤维寡糖和木寡糖的转录响应。
Transcriptional response of the cellobiose dehydrogenase gene to cello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium.
机构信息
Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Appl Environ Microbiol. 2012 May;78(10):3770-3. doi: 10.1128/AEM.00150-12. Epub 2012 Mar 9.
Abstract
Cellobiose dehydrogenase (CDH) gene transcripts were quantified by reverse transcription-PCR (RT-PCR) in cultures of Phanerochaete chrysosporium supplemented with various cello- and xylooligosaccharides in order to elucidate the mechanism of enhanced CDH production in xylan/cellulose culture. Cellotriose and cellotetraose induced cdh expression, while xylobiose and xylotriose induced expression of cellobiohydrolase genes, especially cel7C.
摘要
通过在补充了各种纤维二糖和木低聚糖的黄孢原毛平革菌（Phanerochaete chrysosporium）培养物中进行反转录 PCR（RT-PCR），对纤维二糖脱氢酶（CDH）基因转录本进行了定量，以阐明在木聚糖/纤维素培养物中增强 CDH 产生的机制。纤维三糖和纤维四糖诱导 cdh 表达，而木二糖和木三糖诱导纤维素酶基因，特别是 cel7C 的表达。
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