Chen Yu, Chen Hangrong, Guo Limin, Shi Jianlin
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
J Nanosci Nanotechnol. 2011 Dec;11(12):10844-8. doi: 10.1166/jnn.2011.4047.
Hollow mesoporous silica nanospheres with large pore size of around 11 nm have been synthesized by a structural difference based selective etching strategy, and the highly dispersed hydrophobic Fe3O4 nanoparticles with a particle size of 5 nm were then impregnated into hollow cores of nanospheres through these large pores by a vacuum impregnation technique. The structural characteristics of obtained magnetic composites were characterized by X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Selected Area Electron Diffraction (SAED), Ultraviolet-visible (UV-Vis) and Vibrating Sample Magnetometer (VSM). The results show that the obtained Fe3O4-hollow mesoporous silica composites exhibit superparamagnetic property with saturation magnetization value of 4.17 emu/g. Furthermore, the obtained supports show ultrafast immobilization of hemoglobin and the immobilized enzymes are not denatured, indicating that the superparamagnetic hollow mesoporous silica spheres are excellent support for immobilization of enzymes with magnetic recycling property.
通过基于结构差异的选择性蚀刻策略合成了孔径约为11nm的中空介孔二氧化硅纳米球，然后采用真空浸渍技术通过这些大孔将粒径为5nm的高度分散的疏水性Fe3O4纳米颗粒浸渍到纳米球的中空核中。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、选区电子衍射（SAED）、紫外可见光谱（UV-Vis）和振动样品磁强计（VSM）对所得磁性复合材料的结构特征进行了表征。结果表明，所得Fe3O4-中空介孔二氧化硅复合材料具有超顺磁性，饱和磁化强度值为4.17emu/g。此外，所得载体对血红蛋白具有超快固定作用，且固定化酶未变性，表明超顺磁性中空介孔二氧化硅球是具有磁循环性能的酶固定化的优良载体。
