Rocha Rita Gomes, Leite Yuri Luiz Reis, Ferreira Eduardo, Justino Juliana, Costa Leonora Pires
Departamento de Biologia and CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Mitochondrial DNA. 2012 Apr;23(2):77-83. doi: 10.3109/19401736.2012.660922. Epub 2012 Mar 13.
This study reports the occurrence of highly conserved d-loop sequences in the mitochondrial genome of the woolly mouse opossum genus Marmosa subgenus Micoureus (Mammalia, Didelphimorphia, Didelphidae). Sixty-six sequences of Marmosa (Micoureus) demerarae, Marmosa (Micoureus) constantiae, and Marmosa (Micoureus) paraguayanus were amplified using universal d-loop primers and virtually no genetic differences were detected within and among species. These sequences matched the control region of the mitochondrial marsupial genome. Analyses of qualitative aspects of these sequences revealed that their structural composition is very similar to the d-loop region of other didelphid species. However, the total lack of variability has not been reported from other closely related species. The data analyzed here support the occurrence of highly conserved d-loop sequences, and we found no support for the hypothesis that these sequences are d-loop-like nuclear pseudogenes. Furthermore, the control and flanking regions obtained with different primers corroborate the lack of variability of the d-loop sequences in the mitochondrial genome of Marmosa (Micoureus).
本研究报告了绵毛负鼠属（Marmosa）小绵毛负鼠亚属（Micoureus）（哺乳纲，袋鼬目，负鼠科）线粒体基因组中高度保守的d环序列的存在情况。使用通用d环引物扩增了德氏小绵毛负鼠（Marmosa (Micoureus) demerarae）、康氏小绵毛负鼠（Marmosa (Micoureus) constantiae）和巴拉圭小绵毛负鼠（Marmosa (Micoureus) paraguayanus）的66个序列，在种内和种间几乎未检测到遗传差异。这些序列与有袋类线粒体基因组的控制区相匹配。对这些序列的定性分析表明，它们的结构组成与其他负鼠科物种的d环区域非常相似。然而，其他近缘物种尚未报道完全缺乏变异性的情况。此处分析的数据支持高度保守的d环序列的存在，并且我们没有找到支持这些序列是类似d环的核假基因这一假设的证据。此外，用不同引物获得的控制区和侧翼区证实了小绵毛负鼠线粒体基因组中d环序列缺乏变异性。
