Grupo de Investigación en Acuicultura, University of Las Palmas de Gran Canaria, Instituto Universitario de Sanidad Animal, Trasmontaña s/n, 35413 Arucas, Las Palmas, Canary Islands, Spain.
Br J Nutr. 2012 Dec 28;108(12):2115-28. doi: 10.1017/S0007114512000311. Epub 2012 Mar 13.
The objective of the present study was to determine the effect of Se inclusion in high-DHA and vitamin E microdiets (5 g DHA/100 g dry weight and 300 mg vitamin E/100 g dry weight; 5 g DHA/100 g dry weight and 300 mg vitamin E/100 g dry weight supplemented with Se) in comparison with a control diet (1 g DHA/100 g dry weight and 150 mg vitamin E/100 g dry weight) on sea bass larval growth, survival, biochemical composition, malonaldehyde (MDA) content, muscle morphology and antioxidant enzymes (AOE), insulin-like growth factors (IGF) and myosin expression. For a given DHA and vitamin E dietary content, Se inclusion favoured larval total length and specific growth rate, and reduced the incidence of muscular lesions, MDA contents and AOE gene expression. In contrast, IGF gene expression was elevated in the 5/300 larvae, suggesting an increased muscle mitogenesis that was corroborated by the increase in mRNA copies of myosin heavy chain. The results of the present study denoted the beneficial effect of Se not only in preventing oxidative stress, as a glutathione peroxidase cofactor, but probably due to other as yet unknown physiological functions.
本研究的目的是确定在高 DHA 和维生素 E 微饲料（5g DHA/100g 干重和 300mg 维生素 E/100g 干重；5g DHA/100g 干重和 300mg 维生素 E/100g 干重补充硒）中添加硒与对照组饮食（1g DHA/100g 干重和 150mg 维生素 E/100g 干重）相比，对鲈鱼幼体生长、成活率、生化组成、丙二醛（MDA）含量、肌肉形态和抗氧化酶（AOE）、胰岛素样生长因子（IGF）和肌球蛋白表达的影响。在给定的 DHA 和维生素 E 饮食含量下，添加硒有利于幼体全长和特定生长率，并降低肌肉病变、MDA 含量和 AOE 基因表达的发生率。相比之下，5/300 组的 IGF 基因表达升高，表明肌肉有丝分裂增加，这与肌球蛋白重链 mRNA 拷贝数的增加相符。本研究的结果表明，硒不仅作为谷胱甘肽过氧化物酶的辅助因子对预防氧化应激具有有益作用，而且可能由于其他尚未知的生理功能。
