Sansuwan Kanokwan, Jintasataporn Orapint, Rink Lothar, Triwutanon Supawit, Wessels Inga
Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
Institute of Immunology, Faculty of Medicine, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074 Aachen, Germany.
Biology (Basel). 2023 Feb 21;12(3):338. doi: 10.3390/biology12030338.
Since Asian sea bass is one of the economically most important fish, aquaculture conditions are constantly optimized. Evidence from feeding studies combined with the current understanding of the importance of zinc for growth and immune defense suggest that zinc supplementation may be a possible approach to optimize aquacultures of Asian sea bass. To investigate the effects of zinc deficiency and zinc supplementation, cells from Asian sea bass were incubated in culture medium with different zinc contents. The expression of genes, important for zinc homeostasis, redox metabolism, and growth hormones was analyzed using RT-PCR. Zinc deficiency induced the expression of certain zinc transporters (ZIP14, ZIP10, ZIP6, ZIP4, ZnT4, ZnT9) as well as of SOD1, IGF I and IGF II, while expression of ZnT1 and metallothionein (MT) was reduced. Zinc supplementation decreased the expression of ZIP10, while expression of ZnT1 and MT were elevated. No differences in the effects of zinc supplementation with zinc sulfate compared to supplementation with zinc amino acid complexes were observed. Thus, extracellular zinc conditions may govern the cellular zinc homeostasis, the redox metabolism and growth hormone expression in cells from Asian sea bass as reported for other fish species. Our data indicate that supplementing aquacultures with zinc may be recommended to avoid detriments of zinc deficiency.
由于尖吻鲈是经济上最重要的鱼类之一，其水产养殖条件在不断优化。喂养研究的证据，结合当前对锌在生长和免疫防御方面重要性的理解，表明补充锌可能是优化尖吻鲈水产养殖的一种可行方法。为了研究锌缺乏和补充锌的影响，将尖吻鲈的细胞在含不同锌含量的培养基中培养。使用逆转录聚合酶链反应（RT-PCR）分析对锌稳态、氧化还原代谢和生长激素重要的基因表达。锌缺乏诱导了某些锌转运蛋白（ZIP14、ZIP10、ZIP6、ZIP4、ZnT4、ZnT9）以及超氧化物歧化酶1（SOD1）、胰岛素样生长因子I（IGF I）和胰岛素样生长因子II（IGF II）的表达，而ZnT1和金属硫蛋白（MT）的表达降低。补充锌降低了ZIP10的表达，而ZnT1和MT的表达升高。未观察到硫酸锌补充锌与氨基酸锌络合物补充锌的效果有差异。因此，如其他鱼类物种所报道的那样，细胞外锌条件可能控制尖吻鲈细胞中的细胞锌稳态、氧化还原代谢和生长激素表达。我们的数据表明，建议在水产养殖中补充锌以避免锌缺乏的危害。
