Gao Shan, Wang Xing-hui, Huang Ling-ling, Yu Ting-ting, Du Su-ming, Guo Yan-wei, Jia Yuan, Wang Jian
Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China.
Zhong Xi Yi Jie He Xue Bao. 2012 Mar;10(3):330-6. doi: 10.3736/jcim20120313.
To investigate the effects of Xinji' erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
Isoproterenol was given subcutaneously (1 mg/kg, twice per day for 7 d) to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, XJEK low-, medium- and high- dose groups, XJEK water layer group, XJEK n-butanol layer group and metoprolol group. All drugs were given by intragastric administration. At the end of the 7th day, the hearts of the rats were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight (HW/BW). The histological changes were observed by hemotoxylin-eosin and Van Gieson staining. Colorimetric method was used to determine the content of hydroxyproline in heart, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum.
Compared with the isoproterenol injection only, XJEK potently inhibited cardiomyocyte hypertrophy and the increase of hydroxyproline content in heart (P<0.01), improved cardiac pathology change, inhibited the decrease of SOD activity and the increase of MDA content in serum (P<0.01). XJEK water layer also inhibited the increase of cardiomyocyte hypertrophy (P<0.01) while XJEK n-butanol layer inhibited cardiomyocyte hypertrophy and fibrosis (P<0.01).
XJEK possesses protective effects against isoproterenol-induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body. XJEK water layer and XJEK n-butanol layer attenuated ventricular remodeling without significant oxidative stress state changing, which indicates that a non-antioxidative stress mechanism may exist.
研究复方中药心疾尔康（XJEK）对异丙肾上腺素诱导的小鼠心室重构的影响。
皮下注射异丙肾上腺素（1 mg/kg，每天2次，共7天）诱导小鼠心室重构。将小鼠分为正常对照组、模型组、XJEK低、中、高剂量组、XJEK水层组、XJEK正丁醇层组和美托洛尔组。所有药物均通过灌胃给药。在第7天结束时，称量大鼠心脏重量，心肌肥大指数用心脏重量/体重（HW/BW）表示。通过苏木精-伊红和Van Gieson染色观察组织学变化。采用比色法测定心脏中羟脯氨酸含量、血清中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。
与仅注射异丙肾上腺素相比，XJEK能有效抑制心肌细胞肥大和心脏中羟脯氨酸含量的增加（P<0.01），改善心脏病理变化，抑制血清中SOD活性的降低和MDA含量的增加（P<0.01）。XJEK水层也能抑制心肌细胞肥大的增加（P<0.01），而XJEK正丁醇层能抑制心肌细胞肥大和纤维化（P<0.01）。
XJEK对异丙肾上腺素诱导的小鼠心室重构具有保护作用，这可能与其降低氧化应激和提高机体抗氧化活性的作用有关。XJEK水层和XJEK正丁醇层减轻了心室重构，而氧化应激状态无明显变化，这表明可能存在非抗氧化应激机制。
