巨片形吸虫来源的14.5 kDa脂肪酸结合蛋白在水牛片形吸虫病免疫诊断中的诊断潜力
Diagnostic potential of Fasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis.
作者信息
Allam G, Bauomy I R, Hemyeda Z M, Diab T M, Sakran T F
机构信息
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
出版信息
J Helminthol. 2013 Jun;87(2):147-53. doi: 10.1017/S0022149X12000168. Epub 2012 Mar 13.
The 14.5 kDa fatty acid binding protein (FABP) was isolated from the crude extract of adult Fasciola gigantica worms. Polyclonal anti-FABP IgG was generated in rabbits immunized with prepared FABP antigen. Sandwich enzyme-linked immunosorbent assay (ELISA) was applied to detect coproantigen in stools and circulating Fasciola antigen (CA) in sera of 126 water buffaloes by using purified and horseradish peroxidase (HRP)-conjugated anti-FABP IgG. Sandwich ELISA sensitivity was 96.97% and 94.95%; while specificity was 94.12% and 82.35% for coproantigen and CA detection, respectively. However, sensitivity and specificity of the Kato-Katz technique was 73.74% and 100%, respectively. The diagnostic efficacy of sandwich ELISA was 96.55% and 93.1% for coproantigen and CA detection, respectively. In contrast, the diagnostic efficacy of the Kato-Katz technique was 77.59%. In conclusion, these results demonstrate that the purified 14.5 kDa FABP provides a more suitable antigen for immunodiagnosis of early and current bubaline fascioliasis by using sandwich ELISA.
从成年巨片形吸虫的粗提物中分离出14.5 kDa脂肪酸结合蛋白（FABP）。用制备的FABP抗原免疫兔子，产生多克隆抗FABP IgG。采用夹心酶联免疫吸附测定法（ELISA），使用纯化的辣根过氧化物酶（HRP）偶联抗FABP IgG检测126头水牛粪便中的粪抗原和血清中的循环片形吸虫抗原（CA）。夹心ELISA检测粪抗原和CA的灵敏度分别为96.97%和94.95%；而特异性分别为94.12%和82.35%。然而，加藤厚涂片法的灵敏度和特异性分别为73.74%和100%。夹心ELISA检测粪抗原和CA的诊断效能分别为96.55%和93.1%。相比之下，加藤厚涂片法的诊断效能为77.59%。总之，这些结果表明，纯化的14.5 kDa FABP为采用夹心ELISA免疫诊断早期和当前水牛片形吸虫病提供了更合适的抗原。
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