Department of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil.
Bioresour Technol. 2012 May;112:270-4. doi: 10.1016/j.biortech.2012.02.082. Epub 2012 Feb 24.
Sequential solid-state and submerged cultivation with sugarcane bagasse as substrate for cellulase production by Aspergillus niger A12 was assessed by measuring endoglucanase activity. An unconventional pre-culture with an initial fungal growth phase under solid-state cultivation was followed by a transition to submerged fermentation by adding the liquid culture medium to the mycelium grown on solid substrate. For comparison, control experiments were conducted using conventional submerged cultivation. The cultures were carried out in shake flasks and in a 5-L bubble column bioreactor. An endoglucanase productivity of 57 ± 13 IU/L/h was achieved in bubble column cultivations prepared using the new method, representing an approximately 3-fold improvement compared to conventional submerged fermentation. Therefore, the methodology proposed here of a sequential fermentation process offers a promising alternative for cellulase production.
采用固态和浸没式顺序培养，以甘蔗渣为基质，利用黑曲霉 A12 生产纤维素酶，通过测量内切葡聚糖酶活性进行评估。采用一种非传统的预培养方法，在固态培养中进行初始真菌生长阶段，然后通过将液体培养基添加到在固体基质上生长的菌丝体中，过渡到浸没式发酵。为了进行比较，使用传统的浸没式培养进行了对照实验。在摇瓶和 5-L 鼓泡柱生物反应器中进行了培养。使用新方法制备的鼓泡柱培养物中获得了 57±13IU/L/h 的内切葡聚糖酶生产力，与传统的浸没式发酵相比，提高了约 3 倍。因此，这里提出的顺序发酵方法为纤维素酶生产提供了一种有前途的替代方法。
