Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, PR China.
J Biotechnol. 2012 May 31;159(1-2):108-14. doi: 10.1016/j.jbiotec.2012.02.013. Epub 2012 Mar 3.
Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the potential application of CALB-displaying Pichia pastoris cells for the production of flavor esters. The optimal conditions for flavor esters synthesis by this biocatalyst were determined in 50-ml shake flasks. Under optimized conditions, the synthesis of 12 kinds flavor esters were scaled up in a 5-l batch stirred reactor. Among these, the mole conversions of 10 exceeded 95% after reactions for 4h. In addition, this biocatalyst showed good tolerance for high substrates concentration and excellent operational stability. Repeated use of the cells in 10 batches resulted in an activity loss of less than 10%. Thus, CALB-displaying P. pastoris whole cells are robust biocatalysts with potential commercial application in the large-scale production of flavor esters in non-aqueous media.
南极假丝酵母脂肪酶 B（CALB）已被用作制备许多风味酯的有效催化剂。由于其低成本制备和高酶活性，展示 CALB 的酵母全细胞生物催化剂可能是商业固定化 CALB 的有吸引力的替代品。我们研究了展示 CALB 的巴斯德毕赤酵母细胞在风味酯生产中的潜在应用。通过摇瓶实验确定了该生物催化剂合成风味酯的最佳条件。在优化条件下，在 5L 分批搅拌式反应器中放大合成了 12 种风味酯。其中，10 种的摩尔转化率在反应 4 小时后超过 95%。此外，该生物催化剂对高底物浓度具有良好的耐受性和出色的操作稳定性。在 10 批重复使用细胞后，活性损失小于 10%。因此，展示 CALB 的毕赤酵母全细胞是具有潜在商业应用价值的坚固生物催化剂，可用于非水介质中风味酯的大规模生产。
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