State Key Laboratory of Crop Stress Biology in Arid Areas and College of Science, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China; Attana, SE-11419 Stockholm, Sweden.
Attana, SE-11419 Stockholm, Sweden.
Biosens Bioelectron. 2012 May 15;35(1):200-205. doi: 10.1016/j.bios.2012.02.047. Epub 2012 Feb 28.
A novel approach to the study of molecular interactions on the surface of mammalian cells using a QCM biosensor was developed. For this study, an epidermoid carcinoma cell line (A-431) and a breast adenocarcinoma cell line (MDA-MB-468) were immobilized onto polystyrene-coated quartz crystals. The binding and dissociation between the lectin Con A and the cells as well as the inhibition of the binding by monosaccharides were monitored in real time and provided an insight into the complex avidic recognition of cell glycoconjugates. The real-time lectin screening of a range of lectins, including Con A, DBA, PNA and UEA-I, enabled the accurate study of the glycosylation changes between cells, such as changes associated with cancer progression and development. Furthermore, the kinetic parameters of the interaction of Con A with MDA-MB-468 cells were studied. This application provides investigators in the field of glycobiology with a novel tool to study cell surface glycosylation and may also have impacts on drug discovery.
采用 QCM 生物传感器，开发了一种研究哺乳动物细胞表面分子相互作用的新方法。在这项研究中，将表皮癌细胞系（A-431）和乳腺腺癌细胞系（MDA-MB-468）固定在聚苯乙烯涂覆的石英晶体上。实时监测凝集素 Con A 与细胞之间的结合和解离，以及单糖对结合的抑制作用，深入了解细胞糖缀合物的复杂亲和识别。对一系列凝集素（包括 Con A、DBA、PNA 和 UEA-I）的实时凝集素筛选，使我们能够准确研究细胞之间的糖基化变化，如与癌症进展和发展相关的变化。此外，还研究了 Con A 与 MDA-MB-468 细胞相互作用的动力学参数。该应用为糖生物学领域的研究人员提供了一种研究细胞表面糖基化的新工具，也可能对药物发现产生影响。
