Hardwick R A, Prisco M R, Shah D O
Fenwal Division, Baxter Healthcare Corporation, Santa Ana, California 92705.
Artif Organs. 1990 Oct;14(5):342-7. doi: 10.1111/j.1525-1594.1990.tb02978.x.
An improved magnetic separator has been developed for use in large-scale cell separations. This separation method uses paramagnetic microbeads coated with antibodies that selectively bind to target cells. The magnetic separator attracts the microbead-target cell aggregates and holds these aggregates at its surface while the suspending fluid and nontarget cells flow past. The optimum separator design was determined to be two magnetic assemblies in series along with a peristaltic pump. The assemblies consist of neodymium-iron-boron magnet bars sandwiched between steel bars (magnetic pole pieces). The size and pole spacing of the two magnetic assemblies are designed to be different, so that the first assembly, which captures greater than 99.99% of the microbeads, has good magnetic reach-out and a high magnetic holding force at its surface, while the second assembly has an even higher magnetic holding force at its surface. Studies show that the separator can remove 1 x 10(10) microbeads from a suspension of red blood cells processed at a flow rate of 9 ml/min, so that no microbeads are detected in the effluent.
一种改进的磁选器已被开发用于大规模细胞分离。这种分离方法使用涂有抗体的顺磁性微珠，这些抗体能选择性地结合靶细胞。磁选器吸引微珠 - 靶细胞聚集体，并将这些聚集体固定在其表面，同时悬浮液和非靶细胞流过。经确定，最佳的磁选器设计是串联的两个磁组件以及一个蠕动泵。这些组件由夹在钢条（磁极片）之间的钕铁硼磁棒组成。两个磁组件的尺寸和磁极间距设计成不同的，这样，捕获超过99.99%微珠的第一个组件在其表面具有良好的磁作用范围和高磁保持力，而第二个组件在其表面具有更高的磁保持力。研究表明，该磁选器能够以9毫升/分钟的流速从红细胞悬液中去除1×10¹⁰个微珠，从而在流出物中检测不到微珠。
