Owen C S
Biophys J. 1978 May;22(2):171-8. doi: 10.1016/S0006-3495(78)85482-4.
The high gradient magnetic separation technique has been applied to separate paramagnetic erythrocytes from a cell suspension that also contained diamagnetic cells. Paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state (methemoglobin). Diamagnetic cells were either untreated erythrocytes, containing oxyferrohemoglobin, or leukocytes in a suspension of mouse spleen cells. Cell suspensions were passed through a column containing 40 micron diameter stainless steel wire in a high magnetic field (33 kG). The paramagnetic cells were retained on the surface of the wire while the diamagnetic cells passed through. Elution of the paramagnetic cells was accomplished by removing the column from the magnet, in effect turning off the field.
高梯度磁分离技术已被用于从包含抗磁性细胞的细胞悬液中分离顺磁性红细胞。通过将细胞血红蛋白中的铁原子氧化为三价铁状态(高铁血红蛋白),在红细胞中诱导产生顺磁性。抗磁性细胞要么是未处理的含有氧合亚铁血红蛋白的红细胞,要么是小鼠脾细胞悬液中的白细胞。细胞悬液在高磁场(33 kG)中通过一个装有直径40微米不锈钢丝的柱子。顺磁性细胞被保留在钢丝表面,而抗磁性细胞则通过柱子。通过将柱子从磁体上取下,实际上是关闭磁场,实现了顺磁性细胞的洗脱。