Oyagbemi A A, Odetola A A, Azeez O I
Department of Veterinary Physiology, Biochemistry and Pharmacology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Afr J Med Med Sci. 2010 Dec;39 Suppl:171-8.
This research was designed to investigate the antidiabetic properties of ethanolic extract of Cnidoscolus aconitifolius in alloxan-induced diabetes mellitus in Wistar male albino rats. Thirty male albino rats were used. Diabetes mellitus was induced in five of the six groups (B-F) by a single intra-peritoneal injection at the dose of 100mg/kg after normal fasting blood glucose had been determined. Group A served as the positive control while groups C-E received 100mg/kg, 500mg/kg and 1000mg/kg of Cnidoscolus aconitifolius extract respectively. Group B did not received any treatment while group F received chlorpropamide, a standard drug used in the treatment of diabetes mellitus. Blood glucose and body weights were monitored weekly for four weeks. Plasma lipids and electrolytes such as Total cholesterol, Triglyceride, Low Density Lipoproteins (LDL), High Density Lipoproteins (HDL), Creatinine and Blood Urea Nitrogen (BUN) were determined after four weeks of treatment with Cnidoscolus aconitifolius extract. The results show significant reduction (P<0.001) in the blood glucose in group C (100mg/kg of Cnidoscolus aconitifolius) when compared with diabetic control (Alloxan only) and other treatment groups. There was gradual increase in weight of all treatment groups compared with the diabetic control, which had progressive weight loss. Plasma cholesterol levels also significantly reduced (P<0.001) in rats treated with 1,000mg/kg Cnidoscolus aconitifolius extract. From this study, Cnidoscolus aconitifolius extract was found to considerably reduce blood glucose and plasma cholesterol levels and progressively increase weight gain in diabetic treated rats confirming its traditional use for the treatment of diabetes.
本研究旨在探究麻疯树乙醇提取物对用四氧嘧啶诱导的雄性Wistar白化大鼠糖尿病的抗糖尿病特性。使用了30只雄性白化大鼠。在测定正常空腹血糖后，六组（B - F）中的五组通过腹腔注射100mg/kg的剂量诱导糖尿病。A组作为阳性对照，而C - E组分别接受100mg/kg、500mg/kg和1000mg/kg的麻疯树提取物。B组未接受任何治疗，而F组接受氯磺丙脲，一种用于治疗糖尿病的标准药物。每周监测血糖和体重，持续四周。在用麻疯树提取物治疗四周后，测定血浆脂质和电解质，如总胆固醇、甘油三酯、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、肌酐和血尿素氮（BUN）。结果显示，与糖尿病对照组（仅四氧嘧啶）和其他治疗组相比，C组（100mg/kg麻疯树）的血糖显著降低（P<0.001）。与体重逐渐减轻的糖尿病对照组相比，所有治疗组的体重都逐渐增加。用1000mg/kg麻疯树提取物处理的大鼠血浆胆固醇水平也显著降低（P<0.001）。从这项研究中发现，麻疯树提取物能显著降低糖尿病大鼠的血糖和血浆胆固醇水平，并逐渐增加体重，证实了其在治疗糖尿病方面的传统用途。
