Ballarini Mirko, Frascella Francesca, De Leo Natascia, Ricciardi Serena, Rivolo Paola, Mandracci Pietro, Enrico Emanuele, Giorgis Fabrizio, Michelotti Francesco, Descrovi Emiliano
Department of Applied Sciences and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Opt Express. 2012 Mar 12;20(6):6703-11. doi: 10.1364/OE.20.006703.
In this work we introduce the use of a patterned polymer-based surface functionalization of a one-dimensional photonic crystal (1DPC) for controlling the emission direction of fluorescent proteins (ptA) via coupling to a set of two Bloch Surface Waves (BSW). Each BSW dispersion branch relates to a micrometric region on the patterned 1DPC, characterized by a well defined chemical characteristic. We report on the enhanced and spatially selective excitation of fluorescent ptA, and on the spatially-resolved detection of polarized emitted radiation coupled to specific BSW modes. As a result, we provide an optical multiplexing technique for the angular separation of fluorescence radiated from micrometric regions having different surface properties, even in the case the emitting labels are spectrally identical. This working principle can be advantageously extended to a multi-step nanometric relief structure for self-referencing biosensing or frequency-multiplexed fluorescence detection.
在这项工作中，我们介绍了一种基于图案化聚合物的一维光子晶体（1DPC）表面功能化方法，该方法通过与一组两个布洛赫表面波（BSW）耦合来控制荧光蛋白（ptA）的发射方向。每个BSW色散分支都与图案化1DPC上的一个微米级区域相关，该区域具有明确的化学特性。我们报告了荧光ptA的增强和空间选择性激发，以及与特定BSW模式耦合的偏振发射辐射的空间分辨检测。结果，我们提供了一种光学复用技术，用于对具有不同表面特性的微米级区域发出的荧光进行角度分离，即使发射标记在光谱上相同也是如此。这种工作原理可以有利地扩展到用于自参考生物传感或频率复用荧光检测的多步纳米级浮雕结构。
