Yan W
Institute of Microbiology, Shandong University, Jinan.
Yi Chuan Xue Bao. 1990;17(2):143-7.
This paper reports a recombinant plasmid pSDR12 which is constructed through the substitution of the EcoRI-HindIII fragment of pBR322 by a specific fragment of chromosomal DNA of T. thiooxidans. After it was transformed into C600, the transformants revealed higher levels of Tc resistance. This result shows that a promoter function fragment from autotrophic bacteria is able to express in Escherichia coil.