将 IncP 质粒转移到极端嗜酸硫杆菌中。
Transfer of IncP Plasmids to Extremely Acidophilic Thiobacillus thiooxidans.
机构信息
Institute of Microbiology, Shandong University, Jinan 250100, People's Republic of China.
出版信息
Appl Environ Microbiol. 1992 Jan;58(1):429-30. doi: 10.1128/aem.58.1.429-430.1992.
The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10 to 10 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidans at a frequency of 1.0 x 10 per recipient.
广谱宿主范围的 IncP 质粒 RP4、R68.45、RP1::Tn501 和 pUB307 通过接合直接从大肠杆菌转移到嗜酸硫杆菌 Thiobacillus thiooxidans，频率为每受体 10 到 10 个。研究了 T. thiooxidans 接收和表达抗生素抗性标记的能力。RP4 质粒从 T. thiooxidans 转移回大肠杆菌的频率为每受体 1.0 x 10。
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