Environmental Systems Engineering, University of Regina, Regina, Saskatchewan, Canada.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(6):830-6. doi: 10.1080/10934529.2012.664996.
An analytical method to produce profiles of bacterial biomass fatty acid methyl esters (FAME) was developed employing rapid agitation followed by static incubation (RASI) using selective media of wastewater microbial communities. The results were compiled to produce a unique library for comparison and performance analysis at a Wastewater Treatment Plant (WWTP). A total of 146 samples from the aerated WWTP, comprising 73 samples of each secondary and tertiary effluent, were included analyzed. For comparison purposes, all samples were evaluated via a similarity index (SI) with secondary effluents producing an SI of 0.88 with 2.7% variation and tertiary samples producing an SI 0.86 with 5.0% variation. The results also highlighted significant differences between the fatty acid profiles of the tertiary and secondary effluents indicating considerable shifts in the bacterial community profile between these treatment phases. The WWTP performance results using this method were highly replicable and reproducible indicating that the protocol has potential as a performance-monitoring tool for aerated WWTPs. The results quickly and accurately reflect shifts in dominant bacterial communities that result when processes operations and performance change.
开发了一种分析方法，通过快速搅拌后静态孵育（RASI）利用废水微生物群落的选择性培养基来产生细菌生物量脂肪酸甲酯（FAME）的图谱。将结果编制成一个独特的库，以便在污水处理厂（WWTP）进行比较和性能分析。共分析了来自曝气 WWTP 的 146 个样本，其中包括二次和三级出水的每个样本 73 个。为了进行比较，所有样本均通过相似性指数（SI）进行评估，二级出水的 SI 为 0.88，变异度为 2.7%，三级出水的 SI 为 0.86，变异度为 5.0%。结果还突出了三级和二级出水之间脂肪酸图谱的显著差异，表明在这些处理阶段之间细菌群落的组成发生了相当大的变化。该方法用于 WWTP 的性能结果具有高度可重复性，表明该方案有可能成为曝气 WWTP 的性能监测工具。结果快速准确地反映了工艺操作和性能变化导致的优势细菌群落的变化。
