Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.
Bioorg Med Chem Lett. 2012 Apr 15;22(8):2833-6. doi: 10.1016/j.bmcl.2012.02.078. Epub 2012 Mar 3.
A versatile deep-red fluorescent imaging probe is described that is comprised of a bis(zinc(II)-dipicolylamine) targeting unit covalently attached to a pentamethine carbocyanine fluorophore with Cy5-like spectroscopic properties. A titration assay based on fluorescence resonance energy transfer is used to prove that the probe selectively associates with anionic vesicle membranes whose composition mimics bacterial cell membranes. Whole-body optical imaging experiments show that the probe associates with the surfaces of both Gram-positive and Gram-negative bacteria cells, and it can target the site of bacterial infection in a living mouse. In vivo accumulation at the infection site and subsequent clearance occurs more quickly than a structurally related near-infrared bis(zinc(II)-dipicolylamine) probe. The fact that the same deep-red probe molecule can be used for spectroscopic assays, cell microscopy, and in vivo imaging studies, is an important and attractive technical feature.
一种多功能深红色荧光成像探针，由双（锌（II）-二吡啶甲胺）靶向单元与具有 Cy5 样光谱特性的五甲川碳菁荧光团通过共价键连接而成。基于荧光共振能量转移的滴定实验证明，该探针选择性地与模仿细菌细胞膜组成的阴离子囊泡膜结合。全身光学成像实验表明，该探针与革兰氏阳性和革兰氏阴性细菌细胞的表面结合，并且可以靶向活体小鼠中的细菌感染部位。在感染部位的体内积累和随后的清除比结构上相关的近红外双（锌（II）-二吡啶甲胺）探针更快。同样的深红色探针分子可用于光谱分析、细胞显微镜和体内成像研究，这是一个重要且有吸引力的技术特点。
