Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia.
Bioelectrochemistry. 2012 Oct;87:226-9. doi: 10.1016/j.bioelechem.2012.02.006. Epub 2012 Mar 1.
Chronic kidney disease (CKD) is progressive loss of renal function associated among others with increased intracellular calcium concentration. The purpose of this study was to identify the effects of CKD on cell membrane properties such as human red blood cell Ca(2+) ATPase activity, lymphocyte plasma membrane P2X(7) receptor expression and function. This could help us in elucidating the origin of increased calcium concentration in blood cells. We found out Ca(2+) ATPase activity is decreased in early stage CKD patients resulting in altered calcium removal from cytoplasm. By means of flow cytometry we assessed that P2X(7) receptor expression on lymphocyte membrane is 1.5 fold increased for CKD patients. Moreover, we detected an increased uptake of ethidium bromide through this receptor in CKD at basal conditions. It means CKD lymphocyte membranes contain more receptors which are more permeable thus allowing increased calcium influx from extracellular milieu. Finally, we can state alterations in blood cell membranes are closely linked to CKD and may be responsible for intracellular calcium accumulation.
慢性肾脏病（CKD）是肾功能进行性丧失的一种疾病，与细胞内钙浓度升高有关。本研究旨在确定 CKD 对细胞膜特性的影响，如人红细胞 Ca(2+)ATP 酶活性、淋巴细胞质膜 P2X(7)受体表达和功能。这有助于我们阐明血细胞中钙浓度升高的起源。我们发现，早期 CKD 患者的 Ca(2+)ATP 酶活性降低，导致细胞质中钙的去除发生改变。通过流式细胞术，我们评估了 CKD 患者淋巴细胞膜上 P2X(7)受体的表达增加了 1.5 倍。此外，我们在 CKD 的基础条件下检测到通过该受体的溴化乙锭摄取增加。这意味着 CKD 淋巴细胞膜上的受体数量增加，通透性更高，从而允许细胞外环境中的钙内流增加。最后，我们可以说血细胞膜的改变与 CKD 密切相关，可能是细胞内钙积累的原因。
