Dugan G M, Gumbmann M R
Western Regional Research Center, US Department of Agriculture, Albany, CA 94710.
Food Chem Toxicol. 1990 Aug;28(8):553-9. doi: 10.1016/0278-6915(90)90155-g.
Diets containing 0.8, 2.53 and 8.0% field variety morning glory seed were fed to male and female rats (20 per group) in a 90-day subchronic feeding study. Gross clinical observations, body weight, and feed and water intake were recorded weekly. At 90 days, all surviving rats were autopsied, organs were weighed, and blood chemistry analyses, haematology, and bone-marrow evaluation for evidence of clastogenic effects were performed. Tissues from control (0% seed) and high-dose (8.0% seed) rats were examined histologically. Effects of morning glory seed were noted mainly in the high-dose group of both sexes. These included increases in mortality, feed consumption (on a body-weight basis), water consumption, serum alkaline phosphatase and potassium, white blood cell count, and brain and liver weights (as a percentage of body weight); body-weight gain and serum glucose were decreased. Significant changes seen in high-dose females alone were: increased haemoglobin, serum constituents (urea nitrogen, glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase, and ornithine carbamyl transferase), and organ weights (heart, kidney, spleen and pancreas as a percentage of body weight), and decreases in serum albumin, total protein, albumin:globulin ratio, and calcium. Significant changes occurring in high-dose males alone were: increased testicular weight (as a percentage of body weight), increased serum phosphorus, and decreased serum cholesterol. Liver degeneration in the high-dose females was greater than that in the controls. Mortality at 8.0% seed in the diet was 40% in males and 10% in females. At 0.8% seed, the only parameter that differed significantly from that of the controls was a final body-weight reduction in females without a corresponding reduction in feed consumption.
在一项为期90天的亚慢性喂养研究中，给雄性和雌性大鼠（每组20只）喂食含有0.8%、2.53%和8.0%田间品种牵牛花籽的日粮。每周记录总体临床观察结果、体重以及饲料和水的摄入量。在90天时，对所有存活的大鼠进行尸检，称量器官重量，并进行血液化学分析、血液学检查以及骨髓评估以寻找致断裂效应的证据。对对照组（0%籽）和高剂量组（8.0%籽）大鼠的组织进行组织学检查。牵牛花籽的影响主要在高剂量组的雌雄两性中观察到。这些影响包括死亡率增加、饲料消耗量（按体重计算）、饮水量、血清碱性磷酸酶和钾、白细胞计数以及脑和肝脏重量（占体重的百分比）增加；体重增加和血清葡萄糖降低。仅在高剂量雌性大鼠中观察到的显著变化包括：血红蛋白增加、血清成分（尿素氮、谷丙转氨酶、谷草转氨酶和鸟氨酸氨甲酰转移酶）增加以及器官重量（心脏、肾脏、脾脏和胰腺占体重的百分比）增加，同时血清白蛋白、总蛋白、白蛋白：球蛋白比值和钙降低。仅在高剂量雄性大鼠中发生的显著变化包括：睾丸重量（占体重的百分比）增加、血清磷增加以及血清胆固醇降低。高剂量雌性大鼠的肝脏变性比对照组更严重。日粮中含有8.0%籽时，雄性大鼠的死亡率为40%，雌性大鼠为10%。在含有0.8%籽时，与对照组相比唯一显著不同的参数是雌性大鼠最终体重减轻，而饲料消耗量没有相应减少。
