Toxicological evaluation of jimson weed (Datura stramonium) seed.

Diets containing 0.5, 1.58 and 5.0% jimson weed seed were fed to male and female rats (20/group) in a 90-day subchronic feeding study. The alkaloid content was 2.71 mg atropine and 0.66 mg scopolamine/g of seed. Gross clinical observations, body weights and feed and water intakes were recorded weekly. Tear production and pupil dilation measurements were made throughout the study. At 90 days, all of the animals were autopsied and clinical-chemistry analyses, complete haematology and bone-marrow evaluation for evidence of clastogenic effects were performed. Tissues from control (0% seed) and high-dose animals were examined histologically. The principal effects of jimson weed seed were: decreased body-weight gain, serum albumin and serum calcium; increased liver and testes weights (as a percentage of body weight), serum alkaline phosphatase and blood urea nitrogen. Female rats showed more marked responses to jimson weed seed than did males. In addition to the effects seen in both sexes, the females developed decreased serum total protein and cholesterol, and increased serum glutamic-pyruvic transaminase and chloride, red blood cell count, haemoglobin concentration and packed red cell volume. No histological lesions were associated with ingestion of jimson weed seed at 5.0%. It is concluded that jimson weed seed at concentrations of 0.5% or more in the diet produced adverse physiological changes in rats.