Pathology Department, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Cytotherapy. 2012 May;14(5):621-9. doi: 10.3109/14653249.2012.667875. Epub 2012 Mar 20.
Stem cells are commonly enumerated with bead-based methods in blood and marrow progenitor cell transplantation centers. We compared the International Society of Hematotherapy and Graft Engineering (ISHAGE) bead-based method with a true volumetric one that obviates the use of fluorescent beads for enumeration.
From 31 samples, including 15 peripheral blood samples and 16 leukapheresis products, CD34 (+) cells were enumerated with the single-platform bead-based ISHAGE method and a true volumetric method. After exclusion of two outliers, one from the peripheral blood group and the other from the leukapheresis group, the results were compared.
In the peripheral blood category, no significant difference was observed. However, a proportional systematic error was seen in the leukapheresis group. The systematic error was corrected in the leukapheresis group using a regression line equation. The 95% confidence interval of differences was [-5.83, 2.18] for the peripheral blood and [-38.40, 38.77] for the leukapheresis group after correction of the systematic error.
The true volumetric method is a simple and reliable approach that can be used instead of the more popular bead-based procedures.
干细胞在血液和骨髓祖细胞移植中心通常采用基于微球的方法进行计数。我们比较了国际造血细胞治疗和移植工程学会（ISHAGE）基于微球的方法和一种真正的体积测量方法，后者避免了使用荧光微球进行计数。
从 31 个样本中，包括 15 个外周血样本和 16 个白细胞分离产物，使用单平台基于微球的 ISHAGE 方法和真正的体积测量方法对 CD34(+)细胞进行计数。排除两个离群值后，一个来自外周血组，另一个来自白细胞分离组，对结果进行比较。
在外周血组中，没有观察到显著差异。然而，在白细胞分离组中存在比例系统性误差。使用回归线方程对外周血和白细胞分离组的白细胞分离组中的系统性误差进行了校正。校正后，外周血的差异 95%置信区间为[-5.83, 2.18]，白细胞分离组为[-38.40, 38.77]。
真正的体积测量方法是一种简单可靠的方法，可以替代更流行的基于微球的方法。
