FES-Iztacala, UNAM, Tlalnepantla, México.
Mycoses. 2012 May;55(3):e151-7. doi: 10.1111/j.1439-0507.2012.02188.x. Epub 2012 Mar 19.
To detect the frequency and expression of eight ALS (agglutinin-like sequence) genes and the HWP1 genotype in a group of Candida albicans strains isolated from Mexican women suffering from vaginal candidosis. A group of 264 women (age 15-57 years) with vaginal infections were evaluated. C. albicans was identified by PCR amplification of the rRNA internal transcribed spacer regions ITS1 and ITS2. The ALS and HWP1 genes were identified by conventional PCR, and their expression levels were determined by real-time PCR after growing C. albicans strains in reconstituted human vaginal epithelium (RHVE). C. albicans was identified in 50 women (18.9%). The genotypic frequencies were ALS1 100%, ALS2 60%, ALS3 36%, ALS4 54%, ALS5 70%, ALS6 56%, ALS7 64%, ALS9 66% and HWP1 92%. The most frequently expressed genes in the strains harbouring all of the genes were ALS4 (100%), ALS1 (87.5%), ALS2 (87.5%), ALS3 (87.5%), ALS5 (87.5%), ALS7 (87.5%) and HWP1 (75.0%). Nineteen per cent of the vaginal infections were caused by C. albicans, and a high proportion of the strains carried genes encoding proteins involved in adhesion to epithelia. The ALS and HWP1 genes were expressed in RHVE, suggesting that the Als and Hwp1 proteins play an important role in the pathogenesis of the infection.
为了检测一组分离自患有阴道念珠菌病的墨西哥女性的白色念珠菌菌株中 8 个凝集素样序列（agglutinin-like sequence，ALS）基因和 HWP1 基因型的频率和表达。评估了一组 264 名（年龄 15-57 岁）患有阴道感染的女性。通过聚合酶链反应（PCR）扩增 rRNA 内转录间隔区 ITS1 和 ITS2 来鉴定白色念珠菌。通过常规 PCR 鉴定 ALS 和 HWP1 基因，并在重组人阴道上皮（reconstituted human vaginal epithelium，RHVE）中培养白色念珠菌菌株后，通过实时 PCR 确定其表达水平。在 50 名女性（18.9%）中鉴定出白色念珠菌。基因型频率为 ALS1 100%、ALS2 60%、ALS3 36%、ALS4 54%、ALS5 70%、ALS6 56%、ALS7 64%、ALS9 66%和 HWP1 92%。携带所有基因的菌株中表达最频繁的基因是 ALS4（100%）、ALS1（87.5%）、ALS2（87.5%）、ALS3（87.5%）、ALS5（87.5%）、ALS7（87.5%）和 HWP1（75.0%）。19%的阴道感染是由白色念珠菌引起的，许多菌株携带编码与上皮细胞黏附相关蛋白的基因。ALS 和 HWP1 基因在 RHVE 中表达，提示 Als 和 Hwp1 蛋白在感染的发病机制中发挥重要作用。
