Gursky Richard
FEI Company, Hillsboro, Oregon 97124, USA.
Toxicol Pathol. 2012;40(2):403-10. doi: 10.1177/0192623311434178.
This article presents an overview of microscopy and its ability to assist in understanding what happens in cells and tissues. From the 1960s to 1980s, electron microscopy was the best way to understand cell processes, but the advent in the mid-1980s of light microscopy and the ability to do fluorescence imaging displaced electron microscopy in this area. However, the 21st century has seen several improvements in electron microscopy that, along with the need for more detailed ultrastructural information, make it again very attractive in the study of cells, tissues, and organs, and electron microscopy has resumed its place as the preeminent method in understanding cell processes.
本文概述了显微镜技术及其在协助理解细胞和组织中所发生情况方面的能力。从20世纪60年代到80年代，电子显微镜是了解细胞过程的最佳方法，但20世纪80年代中期光学显微镜的出现以及进行荧光成像的能力，使电子显微镜在这一领域的地位被取代。然而，21世纪电子显微镜有了多项改进，再加上对更详细超微结构信息的需求，使其在细胞、组织和器官研究中再次极具吸引力，电子显微镜已重新成为理解细胞过程的卓越方法。
