Deng Jian-Qiang, Liu Bao-Qin, Cai Ji-Feng, Li Wen-Hui, Long Ren, Hou Yi-Ping
Department of Forensic Medicine, Hainan Medical College, Haikou 570102, China.
Fa Yi Xue Za Zhi. 2012 Feb;28(1):41-3.
To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.
DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.
DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.
The system established in this study is reliable and more testing sensitive for forensic trace evidence.
建立基于简并寡核苷酸引物聚合酶链反应（DOP-PCR）的全基因组扩增检测体系，并探讨其可靠性和灵敏度。
采用荧光标记多重短串联重复序列（STR）扩增及毛细管电泳检测系统对DOP-PCR扩增产物进行检测，以确定DOP-PCR体系的可靠性和灵敏度。
成功建立了DOP-PCR体系，经DOP-PCR预处理后进行STR基因分型检测，其检测灵敏度达到5个细胞（30皮克）。
本研究建立的体系可靠，对法医微量物证检测更灵敏。
