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使用6+1 STR试剂盒和EX-Q20电泳进行快速DNA鉴定

[Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis].

作者信息

Ping Yuan, Zhou Huai-Gu, Xu Yan, Xia Zi-Fang, Zheng Wei-Guo

机构信息

Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.

出版信息

Fa Yi Xue Za Zhi. 2011 Dec;27(6):444-6.

PMID:22393596
Abstract

OBJECTIVE

To establish a rapid STR genotyping method for individual identification.

METHODS

Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared.

RESULTS

6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.

CONCLUSION

6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.

摘要

目的

建立一种用于个体识别的快速STR基因分型方法。

方法

采集200份来自FTA的血液样本。用打孔器采集等量血液,并用两种方法进行分析(6+1 STR试剂盒结合EX-Q20电泳和Sinofiler试剂盒结合POP4电泳)。比较两种方法的耗时和结果。

结果

6+1 STR试剂盒结合EX-Q20电泳方法能够获得所有基因分型结果且用时更短。

结论

6+1 STR试剂盒结合EX-Q20电泳方法用于STR基因分型,结果准确、可靠,该新方法在重大刑事案件大规模人员排查比对中有潜在应用价值,还可提高工作效率。

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