Taylor S J, Jones S A, Haggblad J, Greenfield S A
University Department of Pharmacology, Oxford, U.K.
Neuroscience. 1990;37(1):71-6. doi: 10.1016/0306-4522(90)90193-8.
Acetylcholinesterase is released from dopaminergic cells within the substantia nigra. The functional significance of this phenomenon has been studied in the freely-moving animal by a novel system for measuring acetylcholinesterase release from the substantia nigra "on-line" and in vivo. In the unanaesthetized guinea-pig the amount of acetylcholinesterase released was significantly greater than during anaesthesia, and release occurred in a more pulsatile manner. In addition, release of acetylcholinesterase could be evoked by either pharmacological or physiological manipulations, i.e. (1) a depolarizing concentration of potassium ions administered locally; (2) metamphetamine, administered systematically, which also resulted in increased locomotor activity; (3) drinking behaviour, elicited by presentation of a water bottle. Although all three treatments were accompanied by an increase in acetylcholinesterase release within the substantia nigra, potassium-evoked release did not cause any detectable change in behaviour. It is therefore suggested that release of the protein acetylcholinesterase within the substantia nigra is not necessarily a direct cause of locomotor activity: rather, it reflects diverse sensorimotor events.
乙酰胆碱酯酶从黑质内的多巴胺能细胞释放。通过一种新颖的用于在自由活动动物体内“在线”测量黑质乙酰胆碱酯酶释放的系统，对这一现象的功能意义进行了研究。在未麻醉的豚鼠中，乙酰胆碱酯酶的释放量显著高于麻醉期间，且释放以更具脉冲性的方式发生。此外，乙酰胆碱酯酶的释放可通过药理学或生理学操作诱发，即：（1）局部给予去极化浓度的钾离子；（2）系统给予甲基苯丙胺，这也导致运动活动增加；（3）通过提供水瓶引发饮水行为。尽管所有这三种处理都伴随着黑质内乙酰胆碱酯酶释放的增加，但钾离子诱发的释放并未引起任何可检测到的行为变化。因此，有人提出黑质内蛋白质乙酰胆碱酯酶的释放不一定是运动活动的直接原因：相反，它反映了多种感觉运动事件。
