Mallet E, Basuyau J P, Brunelle P, de Menibus C H
Pediatr Res. 1979 May;13(5 Pt 1):647-8. doi: 10.1203/00006450-197905000-00015.
Changes in plasma adenosine 3'5' (cAMP) and guanosine (cGMP) monophosphate, measured by specific radioimmunoassay, after 150 USP/M2 of bovine parathyroid hormone (bPTH) iv administered were studied in children with pseudohypoparathyroidism, and idiopathic hypoparathyroidism, and in normal controls. Basal concentrations of plasma cAMP (17 nmole/1 +/- 1, 6 SEM) and cGMP (8,7 nmole/1 +/- 1, 3 SEM) were the same in all studied children. Plasma cAMP in normal and idiopathic hypoparathyroid children significantly (30-fold, P less than 0.001) and constantly rose with a peak value (537 nmole/1 +/- 210 SEM) observed 5--10 min after bPTH injection. By contrast, no significant change in plasma cAMP occurred in children with pseudohypoparathyroidism. The data confirmed further the unability of pseudohypoparathyroid children to increase cAMP after exogenous PTH, while the cGMP response did not appear to be significantly modified. It is suggested that an injection of 150 USP/m2 bPTH with plasma samples for cAMP assay taken before and 10 min after hormone administration represents a simplified assessment of Ellsworth-Howard's test.
通过特异性放射免疫分析法，研究了静脉注射150 USP/M2牛甲状旁腺激素（bPTH）后，假性甲状旁腺功能减退症患儿、特发性甲状旁腺功能减退症患儿及正常对照者血浆3',5'-环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）的变化。所有研究患儿血浆cAMP的基础浓度（17纳摩尔/升±1.6，标准误）和cGMP的基础浓度（8.7纳摩尔/升±1.3，标准误）相同。正常及特发性甲状旁腺功能减退症患儿血浆cAMP在注射bPTH后显著（30倍，P<0.001）且持续升高，在注射后5 - 10分钟达到峰值（537纳摩尔/升±210，标准误）。相比之下，假性甲状旁腺功能减退症患儿血浆cAMP无显著变化。这些数据进一步证实了假性甲状旁腺功能减退症患儿在外源性甲状旁腺激素作用下无法增加cAMP，而cGMP反应似乎未发生显著改变。建议在注射150 USP/m2 bPTH前后分别采集血浆样本用于cAMP检测，这代表了一种简化的埃尔斯沃思 - 霍华德试验评估方法。
