波兰马肉瘤中检测到的牛乳头瘤病毒E5的系统发育分析。
Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland.
作者信息
Szczerba-Turek A, Siemionek J, Bancerz-Kisiel A, Raś A, Szweda W
机构信息
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
出版信息
Pol J Vet Sci. 2011;14(4):653-4. doi: 10.2478/v10181-011-0096-6.
The aim of the study was to analyse a part of the sequence of the E5 gene of bovine papillomaviruses (BPV) associated with equine sarcoids in Polish horses. Samples of 40 skin lesions obtained from 29 horses were collected for molecular examination. The PCR amplicons of BPV DNA were detected in 38 specimens. After phylogenetic analysis 37 specimens were recognized as BPV-1 and one as BPV-2. Phylogenetic analysis has allowed the classification of the amplicons into two phylogenetic groups (A1,) and four separate isolates (2, 10, 16, 17).
本研究的目的是分析与波兰马匹的马属肉瘤相关的牛乳头瘤病毒（BPV）E5基因的部分序列。从29匹马身上采集了40个皮肤病变样本用于分子检测。在38个样本中检测到了BPV DNA的PCR扩增产物。经过系统发育分析，37个样本被鉴定为BPV-1，1个样本被鉴定为BPV-2。系统发育分析已将扩增产物分为两个系统发育组（A1）和四个单独的分离株（2、10、16、17）。
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