波兰马皮肤肉瘤中检测到的牛乳头瘤病毒类型的遗传评估。

Genetic evaluation of bovine papillomavirus types detected in equine sarcoids in Poland.

作者信息

Szczerba-Turek A, Siemionek J, Ras A, Bancerz-Kisiel A, Platt-Samoraj A, Lipczynska-Ilczuk K, Szweda W

机构信息

Department of Epizootiology, Faculty of Veterinary Medicine,University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.

Department of Animal Reproduction with a Clinic, Faculty of Veterinary Medicine,University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.

出版信息

Pol J Vet Sci. 2019 Mar;22(1):25-29. doi: 10.24425/pjvs.2018.125602.

DOI:10.24425/pjvs.2018.125602

PMID:30997762

Abstract

BACKGROUND

Equine sarcoids are the most common neoplasms in horses. Bovine papilloma- virus type 1 (BPV-1) is the main viral type identified in equine sarcoids in Europe.

OBJECTIVE

The aim of the present study was to genetically evaluate BPV types based on DNA analyses of the CDS of the L1 gene. The presence of BPV DNA was confirmed by Degenerate Oligonucleotide-Primed Polymerase Chain Reaction (DOP PCR) with FAP59/FAP64 consensus primers.

RESULTS

The DNA was detected in 21/40 (52.5%) of clinically diagnosed sarcoids. More than half of 14 isolates (66.7%) shared 100% homology with BPV-1 Deltapapillomavirus 4 isolate 09 asi UK (Acc. No. MF384289) and 99% nucleotide identity with BPV-1 isolate EqSarc1 (Acc. No. JX678969). A comparison with BPV-1 isolate EqSarc1 revealed one silent mutation in C5827T which did not change the aminoacid codon. The remaining 6 isolates (28.6%) shared 100% nucleotide identity with the BPV-1 (Acc. No. X02346) "wild type" isolate, and 1 isolate (4.8%) demonstrated 99% nucleotide identity with BPV-2 (Acc. No. M20219).

CONCLUSIONS

Variants of BPV-1 isolate EqSarc1 (Acc. No. JX678969) constitute the most prevalent type of BPV-1 in Polish horses.

摘要

背景

马肉瘤是马最常见的肿瘤。1型牛乳头瘤病毒（BPV-1）是欧洲马肉瘤中鉴定出的主要病毒类型。

目的

本研究旨在基于L1基因编码区的DNA分析对BPV类型进行基因评估。通过使用FAP59/FAP64共有引物的简并寡核苷酸引物聚合酶链反应（DOP PCR）确认BPV DNA的存在。

结果

在40例临床诊断的肉瘤中，21例（52.5%）检测到DNA。14株分离株中超过一半（66.7%）与BPV-1 Deltapapillomavirus 4分离株09 asi UK（登录号MF384289）具有100%的同源性，与BPV-1分离株EqSarc1（登录号JX678969）具有99%的核苷酸同一性。与BPV-1分离株EqSarc1比较发现，C5827T有一个沉默突变，未改变氨基酸密码子。其余6株分离株（28.6%）与BPV-1（登录号X02346）“野生型”分离株具有100%的核苷酸同一性，1株分离株（4.8%）与BPV-2（登录号M20219）具有99%的核苷酸同一性。