Institute of Tropical Agriculture, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Animal. 2011 Aug;5(10):1539-45. doi: 10.1017/S1751731111000693.
Twelve male 8-month-old lambs were used in a 6-month feeding experiment to determine the effects of dietary Mo, Mo + S and Zn supplements on the body retention and tissue accumulation of dietary Cu, Zn and Fe. The lambs were divided into four groups of three lambs each and each group was fed ad libitum one of four diets. A control diet was based on palm kernel cake (PKC) and grass hay. Three additional diets were the control supplemented with either Mo or Mo+S or Zn. At 3 months of the experiment, feces and urine were collected and sampled for 6 days. At the end of the experiment (6 months), blood was sampled and then the sheep were slaughtered. The liver and kidney were removed and sampled for chemical analysis. In comparison with the control, each dietary supplement decreased (P<0.05) the Cu concentration in the liver, but only the Mo+S supplement decreased it to a safe range of below 350 μg/g dry matter. This was accompanied by the body retention of dietary Cu of 24.6%, 6.7%, 2.5% and 6.5% for the control, Mo, Mo+S and Zn treatments, respectively. The blood plasma concentration of Cu was decreased (P<0.05) by the Zn supplement, but was not affected by other supplements (P>0.05). It was concluded that from the supplements tested, only Mo+S appeared to be effective in reducing the retention and liver accumulation of the dietary Cu to prevent chronic Cu toxicity in sheep fed PKC-based diets.
十二只 8 月龄雄性羔羊参与了一项为期 6 个月的饲养实验，以确定日粮钼、钼+硫和锌补充剂对日粮铜、锌和铁的体保留和组织积累的影响。羔羊分为四组，每组 3 只，每组自由采食四种日粮中的一种。对照组日粮以油棕仁饼（PKC）和干草为主。另外三种日粮是在对照组的基础上补充钼、钼+硫或锌。实验进行到 3 个月时，收集粪便和尿液并采样 6 天。实验结束时（6 个月），采集血液，然后屠宰绵羊。取出肝脏和肾脏进行化学分析。与对照组相比，每种日粮补充剂都降低了（P<0.05）肝脏中的铜浓度，但只有钼+硫补充剂将其降低到低于 350 μg/g 干物质的安全范围。这伴随着日粮铜的体保留率分别为 24.6%、6.7%、2.5%和 6.5%，用于对照组、钼、钼+硫和锌处理。锌补充剂降低了（P<0.05）血浆中铜的浓度，但其他补充剂（P>0.05）没有影响。因此，从测试的补充剂中，只有钼+硫似乎有效减少了日粮铜的保留和肝脏积累，以防止以 PKC 为基础的日粮喂养的绵羊发生慢性铜毒性。
