College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
Molecules. 2012 Mar 22;17(3):3574-85. doi: 10.3390/molecules17033574.
7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.
7-O-甲基柚皮素从报春属植物中提取，属于黄烷酮类多酚。本研究探讨了 7-O-甲基柚皮素对脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞体外细胞因子产生的抗炎作用。结果表明，10、20 或 40μg/mL 的 7-O-甲基柚皮素预处理可呈剂量依赖性地下调肿瘤坏死因子（TNF-α）、白细胞介素（IL-6）和白细胞介素（IL-1β）。此外，我们还研究了信号转导机制，以确定 7-O-甲基柚皮素如何影响 RAW 264.7 巨噬细胞。通过 Western blot 测定丝裂原活化蛋白激酶（MAPK）和 IκBα的激活。数据表明，7-O-甲基柚皮素可下调 LPS 诱导的 ERK1/2、JNK 和 IκBα磷酸化水平。这些观察结果表明，7-O-甲基柚皮素通过阻断 NF-κB、ERK1/2 和 JNK/MAPKs 激活来调节炎症细胞因子反应。
