Sicińska A, Brzozowska A, Sadowy-Sadowski J, Jaklewicz A, Morawiec M, Roszkowski W
Zakładu Podstaw Zywienia Instytutu Zywienia Człowieka SGGW-AR w Warszawie.
Rocz Panstw Zakl Hig. 1990;41(1-2):63-70.
The purpose of the study was determination of the effect of cellulose on the apparent absorption and tissue concentrations of iron, zinc and copper in growing rats. Male Wistar rats with initial body weight 108 +/- 2 g were fed during 6 weeks isocaloric diets containing 1%, 5% or 10% of dietary fibre. The diets contained about 18% protein (casein and wheat gluten 1:1) and iron 28 mg, zinc 17 mg and copper 4.5 mg per kg of diet. In the 2nd, 4th and 6th weeks of the experiment feaces were collected during 2 days. Mineral components of the diets, cases liver, spleen, kidneys and femur were determined by atomic absorption spectrophotometry. No significant differences were noted in diet intake or in body weight gain between these groups. Increased cellulose content in diet, that is 5% or 10%, reduced significantly the absolute and per cent apparent absorption of copper and its concentration in liver, which indirectly led to disturbances of iron metabolism. As a result, the concentration of haemoglobin was reduced and the hepatic iron reserve was raised. No adverse effect of cellulose was found on the apparent absorption of iron and zinc and on zinc concentration in the studied tissues. The per cent coefficients of apparent absorption of iron decreased during the experiment, while those of zinc and copper increased.
本研究的目的是测定纤维素对生长大鼠铁、锌和铜的表观吸收及组织浓度的影响。初始体重为108±2 g的雄性Wistar大鼠，被喂食含有1%、5%或10%膳食纤维的等热量日粮6周。日粮含有约18%的蛋白质（酪蛋白和小麦面筋1:1），每千克日粮含铁28 mg、锌17 mg和铜4.5 mg。在实验的第2、4和6周，收集2天的粪便。通过原子吸收分光光度法测定日粮、肝脏、脾脏、肾脏和股骨的矿物质成分。这些组之间在日粮摄入量或体重增加方面未观察到显著差异。日粮中纤维素含量增加，即5%或10%，显著降低了铜的绝对表观吸收和百分比表观吸收及其在肝脏中的浓度，这间接导致了铁代谢紊乱。结果，血红蛋白浓度降低，肝脏铁储备增加。未发现纤维素对铁和锌的表观吸收以及所研究组织中的锌浓度有不良影响。实验期间，铁的表观吸收百分比系数下降，而锌和铜的表观吸收百分比系数上升。
