Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Langmuir. 2012 Apr 3;28(13):5729-36. doi: 10.1021/la300660x. Epub 2012 Mar 23.
An oil-in-water Pickering emulsion stabilized by biobased material based on a bacteria-chitosan network (BCN) was developed for the first time in this study. The formation of self-assembled BCN was possible due to the electrostatic interaction between negatively charged bacterial cells and polycationic chitosan. The BCN was proven to stabilize the tetradecane/water interface, promoting formation of highly stable oil-in-water emulsion (o/w emulsion). We characterized and visualized the BCN stabilized o/w emulsions by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). Due to the sustainability and low environmental impact of chitosan, the BCN-based emulsions open up opportunities for the development of an environmental friendly new interface material as well as the novel type of microreactor utilizing bacterial cells network.
本研究首次开发了一种基于细菌-壳聚糖网络（BCN）的生物基材料稳定的油包水 Pickering 乳液。由于带负电荷的细菌细胞与聚阳离子壳聚糖之间的静电相互作用，使得自组装 BCN 的形成成为可能。BCN 被证明可以稳定十四烷/水界面，促进高稳定性油包水乳液（o/w 乳液）的形成。我们通过扫描电子显微镜（SEM）和激光扫描共聚焦显微镜（LSCM）对 BCN 稳定的 o/w 乳液进行了表征和可视化。由于壳聚糖具有可持续性和低环境影响，基于 BCN 的乳液为开发环保型新型界面材料以及利用细菌细胞网络的新型微反应器开辟了机会。
