De la Fuente J, Sánchez M, Pérez C, Lauzurica S, Vieira C, González de Chávarri E, Díaz M T
1Dpto. Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, UCM, Puerta de Hierro, s/n, 28040 Madrid, Spain.
Animal. 2010 Feb;4(2):250-8. doi: 10.1017/S1751731109991108.
To evaluate the effect of stocking density and transport time on physiological responses and meat quality, 72 male suckling lambs were transported by road to the slaughterhouse at three different stocking densities (0.12, 0.20 or 0.25 m2/lamb) and two transport times (5 h or 30 min). Blood samples were collected pre-transport at the farm and after unloading in the slaughterhouse to measure levels of cortisol, creatine kinase (CK) and lactate dehydrogenase (LDH). After slaughter, the weights of the hot carcass, liver and spleen were recorded and pH in Musculus longisimus thoracis et lumborum (L), Musculus semitendinosus (ST) and Musculus psoas major (PM) were determined. Colour, water-holding capacity (WHC), texture and thiobarbituric acid-reactive substances (TBARS) values were measured in samples of L, at 24 h post mortem and after 5 days of ageing. Cortisol and LDH were higher in suckling lambs transported for 30 min than those transported for 5 h. Stocking density did not affect blood parameters studied. Transport time significantly affected some carcass quality parameters, but stocking density had no significant effect. Suckling lambs transported for 5 h had lower liver weights and dressing percentages than those transported for 30 min. Transport time influenced pH values, with lambs subjected to the longer journey showing the lowest pH at 0 h in the three muscles studied, with the lowest final pH in L and PM. The PM lambs transported at high density (0.12 m2/lamb) had the lowest pH at 24 h. Transport time and stocking density did not greatly affect colour and texture parameters. The meat from lambs transported for 30 min had higher WHC than meat from lambs transported for 5 h. Animals transported for longer journeys showed higher lipid oxidation after 5 days of ageing than those transported for 30 min. Loading and initial transport caused significant stress response in suckling lambs, that stress response was reduced over the time course of the journey.
为评估饲养密度和运输时间对生理反应及肉质的影响，72只雄性哺乳羔羊以三种不同饲养密度（0.12、0.20或0.25平方米/只）和两种运输时间（5小时或30分钟）通过公路运输至屠宰场。在农场运输前以及在屠宰场卸载后采集血样，以测量皮质醇、肌酸激酶（CK）和乳酸脱氢酶（LDH）水平。屠宰后，记录热胴体、肝脏和脾脏的重量，并测定胸腰最长肌（L）、半腱肌（ST）和腰大肌（PM）的pH值。在宰后24小时和成熟5天后，测定L样本的颜色、持水力（WHC）、质地和硫代巴比妥酸反应性物质（TBARS）值。运输30分钟的哺乳羔羊的皮质醇和LDH高于运输5小时的羔羊。饲养密度不影响所研究的血液参数。运输时间显著影响一些胴体质量参数，但饲养密度无显著影响。运输5小时的哺乳羔羊的肝脏重量和屠宰率低于运输30分钟的羔羊。运输时间影响pH值，运输时间较长的羔羊在所研究的三块肌肉中在0小时时pH值最低，L和PM的最终pH值最低。高密度（0.12平方米/只）运输的PM羔羊在24小时时pH值最低。运输时间和饲养密度对颜色和质地参数影响不大。运输30分钟的羔羊的肉的WHC高于运输5小时的羔羊的肉。运输时间较长的动物在成熟5天后比运输30分钟的动物表现出更高的脂质氧化。装载和初始运输在哺乳羔羊中引起显著的应激反应，该应激反应在运输过程中随时间而降低。
