Nusblat Alejandro D, Bright Lydia J, Turkewitz Aaron P
Cátedra de Biotecnología y Microbiología Industrial, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (C1113AAD) Buenos Aires, Argentina.
Methods Cell Biol. 2012;109:141-75. doi: 10.1016/B978-0-12-385967-9.00006-2.
The past decade has seen a significant expansion in our understanding of membrane traffic in Tetrahymena thermophila, facilitated by the development of new experimental tools and by the availability of the macronuclear genome sequence. Here we review studies on multiple pathways of uptake and secretion, as well as work on metabolism of membrane lipids. We discuss evidence for conservation versus innovation in the mechanisms used in ciliates compared with those in other eukaryotic lineages, and raise the possibility that existing gene expression databases can be exploited to analyze specific pathways of membrane traffic in these cells.
在过去十年中，随着新实验工具的开发以及大核基因组序列的可得，我们对嗜热四膜虫膜运输的理解有了显著扩展。在此，我们综述了关于摄取和分泌多种途径的研究，以及膜脂代谢方面的工作。我们讨论了纤毛虫与其他真核生物谱系在机制上保守与创新的证据，并提出利用现有基因表达数据库来分析这些细胞中膜运输特定途径的可能性。
