俄罗斯鲟精子运动对阳离子的调节。
Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii.
机构信息
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Zatisi 728/II, Vodnany, Czech Republic.
出版信息
Theriogenology. 2012 Jul 1;78(1):102-9. doi: 10.1016/j.theriogenology.2012.01.025. Epub 2012 Mar 22.
The aim of this study was to investigate the response of Russian sturgeon (Acipenser gueldenstaedtii) sperm to external cations (Na(+), K(+), Ca(2+), and Mg(2+)) and their susceptibility on the induction of motility and swimming behavior. An in vitro spermatozoa motility assay was used by a computer-aided Motion-Analysis system. Sperm motility was inhibited by 60 mM NaCl (140 mOsm/kg) and 0.7 mM KCl solutions ( 21.4 mOsm/kg). The Ca(2+) and Mg(2+) ions were not able to inhibit spermatozoa motility. By contrast, Na(+) within a limited concentration range (between 45 and 55 mm) was able to reverse the inhibitory effect of K(+) at the critical concentration (0.7 mM). Ca(2+) and Mg(2+) were also able to reverse the K(+)-mediated spermatozoa motility restriction at concentrations starting at 0.01 and 0.1 mM, respectively. These results provide evidence for the role of K(+) in suppressing spermatozoa motility, and suggest that Ca(2+), Mg(2+), and possibly Na(+) trigger motility in Russian sturgeon sperm.
本研究旨在探究俄罗斯鲟（Acipenser gueldenstaedtii）精子对外界阳离子（Na(+)、K(+)、Ca(2+)和 Mg(2+)）的反应及其对运动和游泳行为诱导的敏感性。采用计算机辅助运动分析系统进行体外精子活力检测。60 mM NaCl（140 mOsm/kg）和 0.7 mM KCl 溶液（21.4 mOsm/kg）可抑制精子活力。Ca(2+)和 Mg(2+)离子不能抑制精子运动。相比之下，Na(+)在有限的浓度范围内（45 至 55 mM 之间）能够逆转在临界浓度（0.7 mM）下 K(+)的抑制作用。Ca(2+)和 Mg(2+)也能够分别从 0.01 和 0.1 mM 开始浓度逆转 K(+)介导的精子运动限制。这些结果为 K(+)在抑制精子活力中的作用提供了证据，并表明 Ca(2+)、Mg(2+)，可能还有 Na(+)触发俄罗斯鲟精子运动。
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