Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL, Utrecht, The Netherlands.
Vet J. 2012 Aug;193(2):557-60. doi: 10.1016/j.tvjl.2012.02.004. Epub 2012 Mar 23.
A real-time quantitative PCR (qPCR) for detection of the apxIVA gene of Actinobacillus pleuropneumoniae was validated using pure cultures of A. pleuropneumoniae and tonsillar and nasal swabs from experimentally inoculated Caesarean-derived/colostrum-deprived piglets and naturally infected conventional pigs. The analytical sensitivity was 5colony forming units/reaction. In comparison with selective bacterial examination using tonsillar samples from inoculated animals, the diagnostic sensitivity of the qPCR was 0.98 and the diagnostic specificity was 1.0. The qPCR showed consistent results in repeatedly sampled conventional pigs. Tonsillar brush samples and apxIVA qPCR analysis may be useful for further epidemiological studies and monitoring for A. pleuropneumoniae.
一种实时定量 PCR（qPCR）用于检测胸膜肺炎放线杆菌的 apxIVA 基因，该方法使用胸膜肺炎放线杆菌纯培养物以及人工接种的剖腹产/初乳剥夺仔猪的扁桃体和鼻腔拭子和自然感染的常规猪进行了验证。分析灵敏度为 5 个菌落形成单位/反应。与使用接种动物的扁桃体样本进行的选择性细菌检查相比，qPCR 的诊断灵敏度为 0.98，诊断特异性为 1.0。qPCR 在反复采样的常规猪中显示出一致的结果。扁桃体刷拭子样本和 apxIVA qPCR 分析可能有助于进一步的流行病学研究和对胸膜肺炎放线杆菌的监测。
