College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, Canada.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Jun 15;92:225-33. doi: 10.1016/j.saa.2012.01.070. Epub 2012 Feb 24.
To date, there is no study on the relationship between carbohydrate (CHO) molecular structures and nutrient availability of combined feeds in ruminants. The objective of this study was to use molecular spectroscopy to reveal the relationship between CHO molecular spectral profiles (in terms of functional groups (biomolecular, biopolymer) spectral peak area and height intensity) and CHO chemical profiles, CHO subfractions, energy values, and CHO rumen degradation kinetics of combined feeds of hulless barley with pure wheat dried distillers grains with solubles (DDGS) at five different combination ratios (hulless barley to pure wheat DDGS: 100:0, 75:25, 50:50, 25:75, 0:100). The molecular spectroscopic parameters assessed included: lignin biopolymer molecular spectra profile (peak area and height, region and baseline: ca. 1539-1504 cm(-1)); structural carbohydrate (STCHO, peaks area region and baseline: ca. 1485-1186 cm(-1)) mainly associated with hemi- and cellulosic compounds; cellulosic materials peak area (centered at ca. 1240 cm(-1) with region and baseline: ca. 1272-1186 cm(-1)); total carbohydrate (CHO, peaks area region and baseline: ca. 1186-946 cm(-1)). The results showed that the functional groups (biomolecular, biopolymer) in the combined feeds are sensitive to the changes of carbohydrate chemical and nutrient profiles. The changes of the CHO molecular spectroscopic features in the combined feeds were highly correlated with CHO chemical profiles, CHO subfractions, in situ CHO rumen degradation kinetics and fermentable organic matter supply. Further study is needed to investigate possibility of using CHO molecular spectral features as a predictor to estimate nutrient availability in combined feeds for animals and quantify their relationship.
迄今为止，关于碳水化合物（CHO）分子结构与反刍动物配合饲料养分有效性之间的关系，尚无研究报道。本研究旨在利用分子光谱学方法，揭示裸大麦与纯小麦DDGS（无壳大麦与纯小麦 DDGS 的比例分别为 100:0、75:25、50:50、25:75、0:100）五种不同组合比的配合饲料中 CHO 分子光谱谱型（生物分子、生物聚合物的官能团光谱峰面积和高度强度）与 CHO 化学谱型、CHO 亚组分、能量值和 CHO 瘤胃降解动力学之间的关系。评估的分子光谱参数包括：木质素生物聚合物分子光谱谱型（峰面积和高度，区域和基线：约 1539-1504cm(-1)）；结构性碳水化合物（STCHO，峰面积区域和基线：约 1485-1186cm(-1)），主要与半纤维素和纤维素化合物相关；纤维素材料峰面积（中心位于约 1240cm(-1)，区域和基线：约 1272-1186cm(-1)）；总碳水化合物（CHO，峰面积区域和基线：约 1186-946cm(-1)）。结果表明，配合饲料中的官能团（生物分子、生物聚合物）对碳水化合物化学和养分谱型的变化敏感。配合饲料中 CHO 分子光谱特征的变化与 CHO 化学谱型、CHO 亚组分、原位 CHO 瘤胃降解动力学和可发酵有机物供应高度相关。需要进一步研究，以探讨利用 CHO 分子光谱特征作为预测动物配合饲料养分有效性和定量其关系的可能性。
