Graduate Institute of Bioresources, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Am J Bot. 2012 Apr;99(4):e157-9. doi: 10.3732/ajb.1100452. Epub 2012 Mar 23.
Fifteen microsatellite loci were developed in an endangered species, Amentotaxus formosana, and were tested in an additional three species, A. argotaenia, A. yunnanensis, and A. poilanei, to evaluate the population structure for conservation efforts and reconstruct the phylogeographic patterns of this ancient lineage.
Polymorphic primer sets were developed from A. formosana; the number of alleles ranged from two to 10, with an observed heterozygosity ranging from 0 to 0.60. All of the loci were found to be interspecifically amplifiable.
These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct genetic units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.
在濒危物种台湾杉中开发了 15 个微卫星位点，并在另外三个物种台湾杉、云南杉和油杉中进行了测试，以评估种群结构，为保护工作提供参考，并重建这个古老谱系的地理格局。
从台湾杉中开发出多态性引物对；等位基因数从 2 到 10 不等，观测杂合度从 0 到 0.60 不等。所有位点都具有种间扩增能力。
这些多态性和可转移的位点将在未来的研究中具有潜在的应用价值，这些研究将集中于识别物种内的不同遗传单位，并建立密切相关物种的地理格局和物种形成过程。
