Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
Free Radic Res. 2012 Jun;46(6):777-84. doi: 10.3109/10715762.2012.678840. Epub 2012 Apr 10.
WD40 repeat proteins have a variety of functions, such as signal transduction, transcription regulation, cell cycle control, autophagy and apoptosis. WDR26 is a novel protein of WD40 repeat proteins and up-regulated during myocardial cells ischemic preconditioning (IPC) but its role in myocardial cell apoptosis induced by oxidative stress and its subcellular localisation are not clear. So we investigated the subcellular localisation of WDR26 and WDR26 expression in rat myocardial ischaemia-reperfusion injury model and H9c2 cells stimulated by H(2)O(2). The results showed that WDR26 can be located at mitochondria and induced by ischaemia-reperfusion injury and H(2)O(2). Then we examined the effects induced by H(2)O(2) in H9c2 cells WDR26 expression. The results showed that WDR26 expression can inhibit apoptosis induced by H(2)O(2). Further, we demonstrated that WDR26 inhibit cytochrome c release from mitochondria. These founding indicate that WDR26 protects myocardial cells against oxidative stress.
WD40 重复蛋白具有多种功能，如信号转导、转录调控、细胞周期控制、自噬和细胞凋亡。WDR26 是 WD40 重复蛋白的一种新型蛋白，在心肌细胞缺血预处理（IPC）过程中上调，但它在氧化应激诱导的心肌细胞凋亡中的作用及其亚细胞定位尚不清楚。因此，我们研究了 WDR26 的亚细胞定位以及 WDR26 在大鼠心肌缺血再灌注损伤模型和 H2O2 刺激的 H9c2 细胞中的表达。结果表明，WDR26 可位于线粒体，并可被缺血再灌注损伤和 H2O2 诱导。然后，我们检查了 H2O2 在 H9c2 细胞中诱导的 WDR26 表达的影响。结果表明，WDR26 的表达可抑制 H2O2 诱导的细胞凋亡。此外，我们证明 WDR26 抑制细胞色素 c 从线粒体释放。这些发现表明，WDR26 可保护心肌细胞免受氧化应激。
