Biosciences,College of Life and Environmental Sciences, Geoffrey Pope, University of Exeter, Stocker Road, Exeter, UK.
Plant J. 2012 Apr;70(1):96-107. doi: 10.1111/j.1365-313X.2011.04884.x.
Without doubt, GFP and spectral derivatives have revolutionized the way biologists approach their journey toward the discovery of how plant cells function. It is fascinating that in its early days GFP was used merely for localization studies, but as time progressed researchers successfully explored new avenues to push the power of GFP technology to reach new and exciting research frontiers. This has had a profound impact on the way we can now study complex and dynamic systems such as plant endomembranes. Here we briefly describe some of the approaches where GFP has revolutionized in vivo studies of protein distribution and dynamics and focus on two emerging approaches for the application of GFP technology in plant endomembranes, namely optical tweezers and forward genetics approaches, which are based either on the light or on genetic manipulation of secretory organelles to gain insights on the factors that control their activities and integrity.
毫无疑问，GFP 和光谱衍生物彻底改变了生物学家探索植物细胞功能的方式。令人着迷的是，在早期 GFP 仅仅被用于定位研究，但随着时间的推移，研究人员成功地探索了新的途径，将 GFP 技术的力量推向新的令人兴奋的研究前沿。这对我们研究复杂和动态系统（如植物内膜系统）的方式产生了深远的影响。在这里，我们简要描述了 GFP 在蛋白质分布和动力学的体内研究中所带来的一些变革，并重点介绍了 GFP 技术在植物内膜系统中的两种新兴应用方法，即光镊和正向遗传学方法，它们分别基于对分泌细胞器的光或遗传操作，以深入了解控制其活性和完整性的因素。
