Ehrhardt David
Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305, USA.
Curr Opin Plant Biol. 2003 Dec;6(6):622-8. doi: 10.1016/j.pbi.2003.09.014.
Fluorescent proteins are generating fresh insight into plant cell function by providing new opportunities to visualize structure and dynamic events in live cells. Novel and transient structures, such as discreet locations in the nucleus where activated photoreceptor proteins accumulate, have recently been identified with fluorescent protein tags. Fluorescent proteins have also enabled the discovery of new dynamic molecular behaviors, such as the repositioning of cortical microtubules by polymer treadmilling. The early potential of fluorescent proteins to reveal protein interactions in living cells is being realized, as demonstrated in recent studies of transcription factors and signal transduction proteins. A promising new approach to the creation of fluorescent-protein-based biosensors has produced an exciting family of tools for visualizing small sugars, and perhaps will produce a wide variety of other small molecules in the future.
荧光蛋白为可视化活细胞中的结构和动态事件提供了新机会，从而为深入了解植物细胞功能带来了新的视角。最近，通过荧光蛋白标签已鉴定出一些新颖的瞬时结构，例如细胞核中活化光感受器蛋白聚集的离散位置。荧光蛋白还促成了新的动态分子行为的发现，比如通过聚合物踏车运动实现皮层微管的重新定位。荧光蛋白在揭示活细胞中蛋白质相互作用方面的早期潜力正在得以实现，最近对转录因子和信号转导蛋白的研究就证明了这一点。一种基于荧光蛋白创建生物传感器的很有前景的新方法，已经产生了一系列用于可视化小糖的令人兴奋的工具，未来或许还会产生各种各样的其他小分子可视化工具。
