Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China.
Antimicrob Agents Chemother. 2012 Jun;56(6):3318-23. doi: 10.1128/AAC.05995-11. Epub 2012 Mar 26.
The extensive use of antibiotics in medicine, the food industry, and agriculture has resulted in the frequent emergence of multidrug-resistant bacteria, which creates an urgent need for new antibiotics. It is now widely recognized that antimicrobial peptides (AMPs) could play a promising role in fighting multidrug-resistant bacteria. Antimicrobial peptide polybia-CP was purified from the venom of the social wasp Polybia paulista. In this study, we synthesized polybia-CP and studied its action mode of antibacterial activity. Our results revealed that polybia-CP has potent antibacterial activity against both Gram-positive and Gram-negative bacteria. The results from both the real bacterial membrane and the in vitro model membrane showed that polybia-CP is membrane active and that its action target is the membrane of bacteria. It is difficult for bacteria to develop resistance to polybia-CP, which may thus offer a new strategy for defending against resistant bacteria in medicine and the food and farming industries.
抗生素在医学、食品和农业领域的广泛应用导致了多重耐药菌的频繁出现，这就迫切需要新的抗生素。现在人们普遍认为抗菌肽（AMPs）在对抗多重耐药菌方面可能发挥着有前景的作用。抗菌肽 polybia-CP 是从社会性黄蜂 Polybia paulista 的毒液中纯化出来的。在这项研究中，我们合成了 polybia-CP 并研究了它的抗菌活性作用模式。我们的结果表明，polybia-CP 对革兰氏阳性菌和革兰氏阴性菌均具有很强的抗菌活性。来自真实细菌膜和体外模型膜的结果表明，polybia-CP 具有膜活性，其作用靶标是细菌的膜。细菌很难对 polybia-CP 产生耐药性，因此这可能为医学、食品和农业领域对抗耐药菌提供了一种新策略。
