Department of Public Health and Community Medicine, Unit of Forensic Medicine, University of Verona, Verona, Italy.
Electrophoresis. 2012 Feb;33(4):599-606. doi: 10.1002/elps.201100383.
The present work is aimed at investigating the influence of the background electrolyte composition and concentration on the separation efficiency and resolution and mass spectrometric detection of illicit drugs in a capillary zone electrophoresis-electrospray ionization-time of flight mass spectrometry (CZE-ESI-TOF MS) system. The effect of phosphate, borate and Tris buffers on the separation and mass spectrometry response of a mixture of 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, methadone, cocaine, morphine, codeine and 6-monoacetylmorphine was studied, in comparison with a reference ammonium formate separation buffer. Inorganic non-volatile borate and Tris buffers proved hardly suitable for capillary electrophoresis-mass spectrometry (CE-MS) analysis, but quite unexpectedly ammonium phosphate buffers showed good separation and ionization performances for all the analytes tested. Applications of this method to real samples of hair from drug addicts are also provided.
本工作旨在研究背景电解质组成和浓度对毛细管区带电泳-电喷雾电离-飞行时间质谱（CZE-ESI-TOF MS）系统中非法药物分离效率、分辨率和质谱检测的影响。研究了磷酸盐、硼酸盐和 Tris 缓冲液对 3,4-亚甲二氧基苯丙胺、3,4-亚甲二氧基甲基苯丙胺、美沙酮、可卡因、吗啡、可待因和 6-单乙酰吗啡混合物的分离和质谱响应的影响，并与参考甲酸铵分离缓冲液进行了比较。结果表明，无机非挥发性硼酸盐和 Tris 缓冲液不太适合毛细管电泳-质谱（CE-MS）分析，但令人意外的是，磷酸铵缓冲液对所有测试的分析物均表现出良好的分离和离子化性能。还提供了该方法在吸毒者头发实际样品中的应用。
