Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Electrophoresis. 2012 Feb;33(4):622-34. doi: 10.1002/elps.201100402.
In recent years, capillary electrophoresis (CE) has matured to a standard method in medicinal inorganic chemistry. More and more steps of the drug discovery process are followed by CE. However, not only the number of applications has steadily increased but also the variety of used methodology has significantly broadened and, as compared to a few years ago, a wider scope of separation modes and hyphenated systems has been used. Herein, a summary of the newly utilized CE methods and their applications in metallodrug research in the timeframe 2006-2011 is presented, following related reviews from 2003 and 2007 (Electrophoresis, 2003, 24, 2023-2037; Electrophoresis 2007, 28, 3436-3446). Areas covered include impurity profiling, quality control of pharmaceutical formulations, lipophilicity estimation, interactions between metallodrugs and proteins or nucleotides, and characterization and also quantification of metabolites in biological matrices and real-world samples.
近年来，毛细管电泳（CE）已发展成为医学无机化学的标准方法。越来越多的药物发现过程的步骤都采用 CE 进行跟踪。然而，不仅应用的数量稳步增加，而且使用的方法种类也显著扩大，与几年前相比，分离模式和联用系统的范围更广。在此，总结了 2006-2011 年期间在金属药物研究中最新应用的 CE 方法及其应用，相关综述发表于 2003 年和 2007 年（电泳，2003 年，24，2023-2037；电泳 2007 年，28，3436-3446）。涵盖的领域包括杂质分析、药物制剂的质量控制、亲脂性评估、金属药物与蛋白质或核苷酸的相互作用，以及生物基质和实际样本中代谢物的特征和定量。
