Holtkamp Hannah, Grabmann Gerlinde, Hartinger Christian G
School of Chemical Sciences, University of Auckland, Auckland, New Zealand.
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
Electrophoresis. 2016 Apr;37(7-8):959-72. doi: 10.1002/elps.201500502. Epub 2016 Feb 16.
Electrophoretic methods have been widely applied in research on the roles of metal complexes in biological systems. In particular, CE, often hyphenated to a sensitive MS detector, has provided valuable information on the modes of action of metal-based pharmaceuticals, and more recently new methods have been added to the electrophoretic toolbox. The range of applications continues to expand as a result of enhanced CE-to-MS interfacing, with sensitivity often at picomolar level, and evolved separation modes allowing for innovative sample analysis. This article is a followup to previous reviews about CE methods in metallodrug research (Electrophoresis, 2003, 24, 2023-2037; Electrophoresis, 2007, 28, 3436-3446; Electrophoresis, 2012, 33, 622-634), also providing a comprehensive overview of metal species studied by electrophoretic methods hyphenated to MS. It highlights the latest CE developments, takes a sneak peek into gel electrophoresis, traces biomolecule labeling, and focuses on the importance of early-stage drug development.
电泳方法已在金属配合物在生物系统中的作用研究中得到广泛应用。特别是，通常与灵敏的质谱检测器联用的毛细管电泳（CE），已提供了有关金属基药物作用模式的有价值信息，并且最近电泳工具库中又增加了新方法。由于CE与质谱联用技术的改进，应用范围不断扩大，灵敏度通常可达皮摩尔水平，并且分离模式的发展使得能够进行创新的样品分析。本文是之前关于金属药物研究中CE方法综述（《电泳》，2003年，24卷，2023 - 2037页；《电泳》，2007年，28卷，3436 - 3446页；《电泳》，2012年，33卷，622 - 634页）的后续文章，还全面概述了通过与质谱联用的电泳方法研究的金属物种。它突出了CE的最新进展，简要介绍了凝胶电泳，追溯了生物分子标记，并着重强调了药物早期开发的重要性。
