生菜中一种赤霉素信号转导负调控因子DELLA蛋白的异常剪接变体。
An unusual spliced variant of DELLA protein, a negative regulator of gibberellin signaling, in lettuce.
作者信息
Sawada Yoshiaki, Umetsu Asami, Komatsu Yuki, Kitamura Jun, Suzuki Hiroyuki, Asami Tadao, Fukuda Machiko, Honda Ichiro, Mitsuhashi Wataru, Nakajima Masatoshi, Toyomasu Tomonobu
机构信息
Department of Bioresource Engineering, Yamagata University, Tsutuoka, Yamagata, Japan.
出版信息
Biosci Biotechnol Biochem. 2012;76(3):544-50. doi: 10.1271/bbb.110847.
DELLA proteins are negative regulators of the signaling of gibberellin (GA), a phytohormone regulating plant growth. DELLA degradation is triggered by its interaction with GID1, a soluble GA receptor, in the presence of bioactive GA. We isolated cDNA from a spliced variant of LsDELLA1 mRNA in lettuce, and named it LsDELLA1sv. It was deduced that LsDELLA1sv encodes truncated LsDELLA1, which has DELLA and VHYNP motifs at the N terminus but lacks part of the C-terminal GRAS domain. The recombinant LsDELLA1sv protein interacted with both Arabidopsis GID1 and lettuce GID1s in the presence of GA. A yeast two-hybrid assay suggested that LsDELLA1sv interacted with LsDELLA1. The ratio of LsDELLA1sv to LsDELLA1 transcripts was higher in flower samples at the late reproductive stage and seed samples (dry seeds and imbibed seeds) than in the other organ samples examined. This study suggests that LsDELLA1sv is a possible modulator of GA signaling in lettuce.
DELLA蛋白是赤霉素（GA）信号传导的负调节因子，赤霉素是一种调节植物生长的植物激素。在具有生物活性的GA存在的情况下，DELLA与可溶性GA受体GID1相互作用会引发DELLA的降解。我们从生菜中LsDELLA1 mRNA的一个剪接变体中分离出cDNA，并将其命名为LsDELLA1sv。据推测，LsDELLA1sv编码截短的LsDELLA1，其在N端具有DELLA和VHYNP基序，但缺少部分C端GRAS结构域。在GA存在的情况下，重组LsDELLA1sv蛋白与拟南芥GID1和生菜GID1s都发生了相互作用。酵母双杂交试验表明LsDELLA1sv与LsDELLA1相互作用。在生殖后期的花样品以及种子样品（干种子和吸胀种子）中，LsDELLA1sv与LsDELLA1转录本的比例高于所检测的其他器官样品。本研究表明，LsDELLA1sv可能是生菜中GA信号传导的调节因子。
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