American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland 20855, USA.
Transfusion. 2012 Nov;52(11):2427-31. doi: 10.1111/j.1537-2995.2012.03616.x. Epub 2012 Mar 27.
A previous study demonstrated that several in vitro storage properties of apheresis platelets (PLTs) that are isolated by sedimentation against the collection container and subsequently resuspended can benefit from a rest period before continuous agitation. This study examines whether the in vitro storage properties of apheresis PLTs isolated by collection from the buffy coat layer benefit from a rest period before agitation.
Freshly collected apheresis PLTs (Trima, GambroBCT) were divided into five 60-mL aliquots. One aliquot was immediately placed on a flat-bed agitator; the other aliquots were held on a laboratory bench for 1, 2, 4, and 6 hours before continuous agitation. Samples were obtained on Days 1, 5, and 7 for standard in vitro PLT assays. The experiment was repeated 12 times.
For each sampling day, no significant differences were observed in aliquots held with or without a rest period for any of the following PLT properties: PLT content, mean PLT volume, pH, pCO2, bicarbonate, glucose, lactate, hypotonic shock response, extent of shape change, aggregation, morphology, CD62P, CD63, and CD42b. Although regression analysis identified several in vitro properties whose mean levels appeared to improve with increasing length of the rest period, maximum differences in mean levels were small (<6%).
The in vitro storage properties of Trima apheresis PLTs isolated from the buffy coat layer do not benefit from a rest period.
先前的一项研究表明，通过沉淀分离收集容器中的血小板（PLT）并随后再悬浮，可使分离的血小板在连续搅拌前有一段休息期，从而改善其多项体外储存特性。本研究旨在探讨从血小板上层白膜层收集的血小板，在搅拌前是否有一段休息期会受益于其体外储存特性。
新鲜采集的血小板（GambroBCT 公司的 Trima 采集系统）被分成 5 份 60 毫升的等分试样。一份立即置于平板搅拌器上；另一份等分试样在实验台上静置 1、2、4 和 6 小时后再进行连续搅拌。在第 1、5 和 7 天取样，进行标准的血小板体外检测。实验重复 12 次。
在每个采样日，与未静置相比，静置 1、2、4 和 6 小时的血小板在以下任何一项血小板特性中均无显著差异：血小板含量、平均血小板体积、pH 值、pCO2、碳酸氢盐、葡萄糖、乳酸、低渗休克反应、形态变化程度、聚集、形态学、CD62P、CD63 和 CD42b。尽管回归分析确定了几种体外特性的平均水平似乎随着静置时间的延长而有所改善，但平均水平的最大差异较小（<6%）。
从血小板上层白膜层分离的 Trima 血小板在体外储存特性上并没有因静置而获益。
