Divisions of Laboratory Medicine and Hematology, Children's National Medical Center, George Washington University School of Medicine and Health Sciences, Washington, DC 20010, USA.
Transfusion. 2011 Dec;51(12):2642-6. doi: 10.1111/j.1537-2995.2011.03230.x. Epub 2011 Jul 11.
To facilitate volume control in neonates, platelets (PLTs) are aliquoted and stored for short periods in non-gas-permeable syringes before infusion. Although agitation of PLTs during storage in gas-permeable bags is performed to maintain their quality, the effect of syringe agitation during storage is unknown.
Double apheresis PLTs (n = 6) were collected and split, providing two identical products. On Days 2 and 4 of storage, aliquots from one bag of each pair were transferred to two syringes and stored for 6 hours on flatbed agitator or were left at 20 to 24 °C without agitation. A series of in vitro tests was performed on Days 0, 2 (Hours 0 and 6), and 4 (Hours 0 and 6). Control samples were obtained from the second matched bag that was stored on the agitator. Data were analyzed by one-way analysis of variance with differences considered significant if the p value was less than 0.05.
Comparable results for several PLT variables were obtained with or without agitation of the syringes. On Day 4 Hour 6, pH values were 7.18 ± 0.12 (agitated syringes) and 7.19 ± 0.1 (nonagitated syringes), and extent of shape change and hypotonic shock response measurements were not significantly different between agitated syringes and nonagitated syringes (23.7 ± 6.4 and 74.3 ± 9.8% vs. 23.3 ± 5.4 and 76.0 ± 7.6%), respectively.
Based on in vitro testing, apheresis PLT aliquots can be stored in syringes for at least 6 hours without agitation before transfusions.
为了便于新生儿的容量控制，血小板（PLT）在输注前被分装并储存在非透气注射器中进行短期储存。尽管在透气袋中储存时会对 PLT 进行搅拌以维持其质量，但在储存过程中注射器搅拌的效果尚不清楚。
采集并分离了双份单采血小板（n = 6），提供了两个完全相同的产品。在储存的第 2 天和第 4 天，将每对袋子中的一份等分试样转移到两个注射器中，在平板搅拌器上储存 6 小时，或在 20 至 24°C 下不搅拌。在第 0 天、第 2 天（第 0 小时和第 6 小时）和第 4 天（第 0 小时和第 6 小时）进行了一系列体外测试。对照样品取自在搅拌器上储存的第二个匹配袋子。通过单向方差分析对数据进行分析，如果 p 值小于 0.05，则认为差异具有统计学意义。
在注射器中进行或不进行搅拌，均可获得几个 PLT 变量的相似结果。在第 4 天第 6 小时，pH 值分别为 7.18 ± 0.12（搅拌注射器）和 7.19 ± 0.1（未搅拌注射器），形状变化和低渗休克反应测量值在搅拌注射器和未搅拌注射器之间无显著差异（分别为 23.7 ± 6.4%和 74.3 ± 9.8%与 23.3 ± 5.4%和 76.0 ± 7.6%）。
根据体外测试，在输注前，APHERESIS PLT 等分试样可在注射器中不搅拌储存至少 6 小时。
